Deleted
Experience: None
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Part:BBa_K1879002

Designed by: Madeleine Stone   Group: iGEM16_Virginia   (2016-10-12)


mutant leucyl synthetase + terminator

This part contains a mutated leucyl-tRNA synthetase (leuS) gene, and a terminator (BBa_J61048). The synthetase gene has been reengineered to contain the necessary mutations for the synthetase to use CBZ-leucine and not normal leucine when charging leucyl-tRNA. The catalytic site of the enzyme has been modified to be complementary to CBZ-leucine rather than leucine, and the editing site has been modified to be non complementary to CBZ-leucine while being complementary to normal leucine. This mutated leuS part is necessary for charging leucyl-tRNA synthetase with CBZ-leucine, thereby generating a dependency on CBZ-leucine for the purposes of containing the genetically engineered organism. The strength of expression of this part can be varied in the organism by adding a promoter and RBS of a desired strength. This allows for easy manipulation of this part by future teams to best serve their purposes.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal AgeI site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


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